Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

30 April 1999

Influence of immunosupressive drugs on liver regeneration after partial hepatectomy in rats - experimental studies

Wojciech G. Polak

Med Sci Monit 1999; 5(3): BR405-409 :: ID: 504729

Abstract

The aim of this study was to asses the influence of selected immunosuppressants on liver regeneration in rats. Material and methods: In sixty male Buffalo strain rats partial hepatectomy (60-65%) was carried out according to Higgins and Anderson under ether anaesthesia. The observation of the rats lasted 6 weeks and 42 days after operation all animals were sacrificed. Rats were randomly assigned to one of six group (10 rats per group) and treated as follows: group 1 - without immunosuppressant (control group), group 2 - cyclosporin A, group 3 - azathioprine, group 4 - tacrolimus, group 5 - prednisolone, group 6 - triple drug therapy (TDT) - azathioprine with cyclosporin A and prednisolone. All rats in each group had following laboratory investigations in blood serum: aspartate aminotransferase (AspAT) activity, alanine aminotransferase (AlAT) activity and alkaline phosphatase (AP) activity. The above parameters were assessed one day before operation and then in 21st and 42nd day after hepatectomy. After 42 days observation all rats were killed and the remnant liver were removed for investigation of hepatic restoration and histologic features. Histologic examination of all sections was performed in light microscope. Furthermore resected liver tissue was weighed the day of operation and also the remnant liver was weighed after sacrification of the animal to calculate the regenerative rate. When applicable the comparison for statistical significance was performed according to the Student's test, Scheffe's test and analysis of variance. A p value of<0.05 was considered to represent a significant difference. Result and conclusions. Regenerative rate of liver tissue was significantly higher in rats treated with cyclosporin A and tacrolimus and significantly lower in rats treated with prednisolone and TDT. There were no significant difference between control group and rats treated with azathioprine. All immunosupressants used in this study influenced on liver function parameters as AspAT, AlAT and AP activity, but the changes were temporary. Histologic examination revealed in 3 rats treated with cyclosporin A restoration nodules with hepatocytes regeneration. In two rats treated with azathioprine there was observed adenocarcinoma coming from bile ducts. One rat treated with tacrolimus presented acute, diffuse necrosis of central parts of hepatic lobules. This study has shown that among immunosupressant used in this experiment only cyclosporin A and tacrolimus stimulated liver regeneration after partial hepatectomy. On the other hand prednisolone and triple drug therapy inhibited liver regeneration, whereas azathioprine had no influence on liver restoration. In some rats treated with cyclosporin A restoration nodules with hepatocytes regeneration occured. In rats receiving azathioprine there were observed two cases of cholangiocarcinoma.

Keywords: Rat, histological examination, regenerative rate, regenerative rate, Tacrolimus, Steroids, Steroids, azathioprine

Add Comment 0 Comments

Editorial

01 May 2024 : Editorial  

Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)

Dinah V. Parums

DOI: 10.12659/MSM.944927

Med Sci Monit 2024; 30:e944927

In Press

17 Mar 2024 : Clinical Research  

Protective Role of TRPC3 Gene Polymorphism (rs10518289) in Obstructive Sleep Apnea Hypopnea Syndrome Among ...

Med Sci Monit In Press; DOI: 10.12659/MSM.942667  

0:00

27 Mar 2024 : Clinical Research  

Impact of Patient Education Formats on Treatment Adherence and Denture Hygiene in Edentulous Patients: A Co...

Med Sci Monit In Press; DOI: 10.12659/MSM.944175  

27 Mar 2024 : Clinical Research  

Incidence, Risk Factors, and Outcomes of Left Atrial Enlargement in Patients Requiring Right Ventricular Pa...

Med Sci Monit In Press; DOI: 10.12659/MSM.944114  

15 May 2024 : Database Analysis  

MRTO4 Enhances Glycolysis to Facilitate HCC Progression by Inhibiting ALDOB

Med Sci Monit In Press; DOI: 10.12659/MSM.944685  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750