Morphological changes of aortal smooth muscle cells exposed to calcitriol in culture
Cecylia Tukaj, Jolanta Kubasik-Juraniec, Michał KraszpulskiMed Sci Monit 2000; 6(4): BR668-674 :: ID: 508115
Abstract
Background: A number of observations suggest that vitamin D plays an important role in maintaining normal cardiovascular function, through its receptors in cardiac muscle or in aortal smooth muscle cells (SMCs). The purpose of this study was to determine the effect of vitamin D active metabolite - calcitriol [1,25(OH)2D3/sub>] on aortal SMCs.
Material/Methods: The cells were isolated from the aortal media of newborn rats by enzymatic digestion and maintained for 6 weeks in primary culture. 1.2 µM of calcitriol was added to the medium every second day.
Results: Light microscopic investigations revealed that the control SMCs formed multilayer with characteristic 'hills and valleys'. Uniform cellular growth patterns were observed after calcitriol treatment. Ultrastructural studies indicated more rapid modulation of SMCs from the 'contractile' to the 'synthetic' phenotype following calcitriol treatment. Elastic fibers were more abundant in treated than in control cultures. The scanning electron microscope showed an increase of regular microvilli on the surface of SMCs. Morphometrical analysis of nuclei demonstrated phenotypic heterogeneity in populations of aortal SMCs. These results suggest that 1) calcitriol can induce changes in the phenotype and in the growth pattern of aortal SMCs, which may be associated with the onset or progression of the atherosclerosis process, 2) the basis of phenotypic changes is related to the stimulation of proliferative activity in these cells.
Keywords: electron microscopy, atherosclerosis, Calcitriol, Culture
582 8
Editorial
01 June 2023 : Editorial
Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic PreparednessDOI: 10.12659/MSM.941209
Med Sci Monit 2023; 29:e941209
In Press
01 Jun 2023 : Clinical Research
Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...Med Sci Monit In Press; DOI: 10.12659/MSM.940472
01 Jun 2023 : Clinical Research
Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s DiseaseMed Sci Monit In Press; DOI: 10.12659/MSM.940873
01 Jun 2023 : Clinical Research
Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...Med Sci Monit In Press; DOI: 10.12659/MSM.940187
01 Jun 2023 : Clinical Research
Influence of Insulin Resistance on Diabetes NephropathyMed Sci Monit In Press; DOI: 10.12659/MSM.939482
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952