Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 January 2008

Differential and opposed transcriptional effects of interleukin-18 gene polymorphisms (-137, +113, and +127) in human HepG-2, HeLa, U937, and THP-1 cells

Xiao Hui LiangABCDEF, Cheung WaiACDEFG, De Yun WangACDEFG

Med Sci Monit 2008; 14(1): BR8-13 :: ID: 636052


Polymorphisms in the IL-18 promoter region (-137, +113, and +127), with perfect linkage disequilibria (Delta=1, p<0.001) among them, were observed in Singaporean Chinese, Malays, and Indians. These polymorphisms appear to have no association with atopic phenotypes. However, functional studies indicate that IL-18 gene polymorphisms strongly affect its activity in cultured cells. The reasons for such conflicting results remain to be determined. It is likely that they are related to the responding cells or to the varying cytokine milieus in the course of the atopic diseases. In this study the effects of IL-18 gene polymorphisms in various IL-18-producing cells were investigated.
Material and Method
Three observed alleles were cloned and transfected into HepG-2, HeLa, U937, and THP-1 cells. The transfectional activities of the cultured cells were analyzed.
Inserted fragments had significant, but opposite transfectional activity in HepG-2 and HeLa cells, while there was no difference in transfectional activity in U937 and THP-1 cells.
These data support the authors' previous observation in which they were unable to detect an association between IL-18 gene polymorphisms and atopic phenotypes in this population. This might be due to different regulatory elements that affect the functional assessment of polymorphisms in different IL-18-producing cells. The functional significance of genetic variants in experimental studies may not be consistent due to complex human traits, but it can also be due to variations in the experimental approaches used, such as the use of different cell or tissue types.

Keywords: Transcription, Genetic, Promoter Regions, Genetic, Polymorphism, Single Nucleotide, Polymorphism, Genetic, Interleukin-18 - genetics, Phenotype, Hypersensitivity, Immediate - immunology, Hela Cells, Haplotypes, Genotype, U937 Cells, Cell Line, Asian Continental Ancestry Group - genetics, 5' Untranslated Regions

Add Comment 0 Comments

601 4


01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209

In Press

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

01 Jun 2023 : Clinical Research  

Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.940873  

01 Jun 2023 : Clinical Research  

Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.940187  

01 Jun 2023 : Clinical Research  

Influence of Insulin Resistance on Diabetes Nephropathy

Med Sci Monit In Press; DOI: 10.12659/MSM.939482  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750