29 August 2008
p53 codon 72 polymorphism and HPV status in lung cancer
Nur BuyruADEFG, Julide AltinisikBC, Mustafa IsinBC, Nejat DalayAEFMed Sci Monit 2008; 14(9): CR493-497 :: ID: 867968
Abstract
Background
Several risk factors, both environmental and genetic, have been associated with the pathogenesis of lung cancer. A common polymorphism at codon 72 of exon 4 of the p53 gene encoding either an arginine or proline has been shown to confer susceptibility to the development of different human malignancies. This polymorphism affects proteolytic degradation of p53 promoted by the HPV-E6 protein and represents a risk factor for human-papillomavirus-induced carcinogenesis.
Material and Method
A total of 65 patients with lung cancer were recruited into the study and 87 healthy subjects were used as the control group. The codon 72 polymorphism was determined by the PCR-RFLP method. Presence of human papillomavirus DNA was analyzed by polymerase chain reaction using MY09 and MY11 consensus primers and hybridization with HPV16 and HPV18 specific probes.
Results
The frequencies of the Arg and Pro alleles were 68.46% and 31.54% in lung cancer patients and 55.7% and 44.3% in the healthy control subjects, respectively. These differences were statistically significant. Human papillomavirus DNA was detected in the blood circulation of only 1 patient.
Conclusions
Our results suggest that the Arg/Arg genotype may represent a potential risk factor for the development of lung cancer independent of the HPV infection.
Keywords: Polymorphism, Genetic, Repressor Proteins - genetics, Papillomavirus Infections - pathology, Oncogene Proteins, Viral - genetics, Lung Neoplasms - virology, Genotype, Genetic Predisposition to Disease, Gene Frequency, Tumor Suppressor Protein p53 - genetics, DNA-Binding Proteins - genetics, DNA, Viral - blood, Codon - genetics, Aged, 80 and over
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
05 Mar 2024 : Clinical Research
Role of Critical Shoulder Angle in Degenerative Type Rotator Cuff Tears: A Turkish Cohort StudyMed Sci Monit In Press; DOI: 10.12659/MSM.943703
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952