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30 March 2009

Evaluation of antibodies against hydatid cyst fluid antigens in the post-treatment follow-up of cystic echinococcosis patients

Tuncay CelikABDEFG, Gulay Aral AkarsuDEFG, Cigdem GungorAEG, Cemil ColakB, Necati OrmeciC

Med Sci Monit 2009; 15(4): CR170-176 :: ID: 869617


Cystic echinococcosis (CE), caused by the metacestode stage of Echinococcus granulosus, is one of the most important zoonoses worldwide. Long post-treatment follow-up is required because of possible relapse. The objective of this study was to determine the values of different antigenic fractions of sheep hydatid cyst fluid in the follow-up of CE patients after treatment.
Material and Method
After gradient gel electrophoresis of sheep hydatid cyst fluid, 45 post-treatment (1 month -16 years) serum samples of CE patients treated with PAIR (puncture, aspiration, injection, and reaspiration) and five post-treatment (1 month) sera of operated CE patients were studied using Western blot. Twenty healthy individuals and five patients infected with other helminths served as controls.
Antigens with molecular weights of 6.5-8, 14, 20, 29, 45, 50, 66, 116-120, 205, and 215 kDa were identified. The bands detected at the highest frequency were 29 kDa (10.4%), 45 kDa (17.2%), and 66 kDa (12.8%). Sera of the 20 healthy controls recognized no specific bands. All of the sera of the five patients with other parasitic diseases recognized one or more hydatid cyst fluid antigen. There was a statistically significant difference between the time after treatment and band weights (p<0.001).
The protein bands of 29 and 205-215 kDa may be valuable in the follow-up of cystic echinococcosis patients as they disappear one year and six months, respectively, after therapy.

Keywords: Electrophoresis, Polyacrylamide Gel, Case-Control Studies, Echinococcosis - immunology, Blotting, Western, Antigens, Protozoan - immunology, Antibodies, Protozoan - immunology

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750