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28 April 2010

Vitamin E succinate (VES) inhibits cell growth and induces apoptosis by mitochondrial-derived ROS in SGC-7901 cells

Li JiaABCDEF, Xiao-Li HuangBD, Yan ZhaoG, Xu-Guang ZhangDG, Kun WuADFG

Med Sci Monit 2010; 16(5): BR131-139 :: ID: 878535


Many reports investigated the effects of vitamin E succinate (VES) on cell growth inhibition and apoptosis in SGC-7901 cells, but the mechanisms are still unclear. This study assessed the effects of VES-induced oxidative stress on apoptosis in SGC-7901 cells and further explored its mechanism.
Material and Method
Cell growth and survival were detected by the MTT assay. Apoptosis was examined by DAPI fluorescence and an Annexin V-FITC apoptosis detection kit. The fluorescent probe CMXRos was used to analyze mitochondrial transmembrane potential by confocal microscopy. Accumulation of intracellular ROS was detected with DCFH-DA. DNA damage was evaluated by the comet assay in SGC-7901 cells. The total GSH concentration was detected by a total glutathione quantification kit. The expression level of Bcl-2 in response to VES treatment was determined by Western blot analysis.
The results showed that VES induced growth inhibition and apoptosis in SGC-7901 cells as assessed by the MTT assay and apoptosis detection method. VES also induced a concentration-dependent generation of reactive oxygen species (ROS) and a concentration-dependent depletion of glutathione (GSH) in the cells. Mannitol, an inhibitor of mitochondrial oxidative damage, prevented ROS generation, GSH depletion, and apoptosis in response to VES in SGC-7901 cells.
VES abrogated the generation of intracellular ROS and the induction of apoptosis. The results indicate that VES induced apoptosis in SGC-7901 cells and this involved mitochondria, likely through mitochondrial- derived ROS.

Keywords: Reactive Oxygen Species - metabolism, Mitochondria - metabolism, Membrane Potentials, Microscopy, Confocal, Glutathione - metabolism, Cell Line, Cell Division - drug effects, Antioxidants - pharmacology, Vitamin E - pharmacology

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750