Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 June 2010

The establishment and characterization of adriamycin-resistant cell lines derived from Saos-2

Bao-Hua NiuACG, Jian-Jun WangB, Yan XiB, Xin-Ying JiADEFG

Med Sci Monit 2010; 16(6): BR184-192 :: ID: 880607


Background: The aim was to establish and characterize adriamycin (ADM)-resistant cell lines.
Material/Methods: Two cell lines, named Saos-2/ADM1and Saos-2/ADM4, resistant to ADM, were established after 167 days.
Results: The resistance indices of methotrexate for the Saos-2/ADM1 and Saos-2/ADM4 cell lines to ADM were 49.8 and 74.6 times higher, respectively, than that of Saos-2. The two cells lines had resistance to MTX, IFO, EPI, THP, and PTX, while the cells were found to remain sensitive to cisplatin. Disordered and coenocytic structure were observed via microscopy.
Conclusions: This is the first report on ADM-resistant cells based on clinical peak doses in serum. MDR1 and MRP genes are involved in the resistance of cell lines to ADM, which are invaluable tools to study the resistance of anticancer drugs and to find the means to revert the resistance.

Keywords: Reverse Transcriptase Polymerase Chain Reaction, Osteosarcoma - metabolism, Methotrexate - pharmacology, Inhibitory Concentration 50, Flow Cytometry - methods, Drug Screening Assays, Antitumor, Drug Resistance, Neoplasm, Doxorubicin - pharmacology, Cisplatin - pharmacology, Antineoplastic Agents - pharmacology, Tetrazolium Salts - pharmacology, Thiazoles - pharmacology

Add Comment 0 Comments

838 56


01 December 2023 : Editorial  

Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory Infections

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.943312

Med Sci Monit 2023; 29:e943312

In Press

01 Dec 2023 : Clinical Research  

Risk Factors and Clinical Outcomes of COVID-19 Infection in Multiple Sclerosis Patients: A Retrospective St...

Med Sci Monit In Press; DOI:  

30 Nov 2023 : Review article  

Decoding the Neurological Sequelae of General Anesthesia: A Review

Med Sci Monit In Press; DOI:  

30 Nov 2023 : Clinical Research  

Enhanced Pain Relief and Muscle Growth in Individuals with Low Back Instability: The Impact of Blood Flow R...

Med Sci Monit In Press; DOI:  

29 Nov 2023 : Clinical Research  

Comparative Analysis of Intramedullary Nail versus Plate Fixation for Fibula Fracture in Supination Externa...

Med Sci Monit In Press; DOI:  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750