Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 June 2010

The establishment and characterization of adriamycin-resistant cell lines derived from Saos-2

Bao-Hua NiuACG, Jian-Jun WangB, Yan XiB, Xin-Ying JiADEFG

Med Sci Monit 2010; 16(6): BR184-192 :: ID: 880607

Abstract

Background: The aim was to establish and characterize adriamycin (ADM)-resistant cell lines.
Material/Methods: Two cell lines, named Saos-2/ADM1and Saos-2/ADM4, resistant to ADM, were established after 167 days.
Results: The resistance indices of methotrexate for the Saos-2/ADM1 and Saos-2/ADM4 cell lines to ADM were 49.8 and 74.6 times higher, respectively, than that of Saos-2. The two cells lines had resistance to MTX, IFO, EPI, THP, and PTX, while the cells were found to remain sensitive to cisplatin. Disordered and coenocytic structure were observed via microscopy.
Conclusions: This is the first report on ADM-resistant cells based on clinical peak doses in serum. MDR1 and MRP genes are involved in the resistance of cell lines to ADM, which are invaluable tools to study the resistance of anticancer drugs and to find the means to revert the resistance.

Keywords: Reverse Transcriptase Polymerase Chain Reaction, Osteosarcoma - metabolism, Methotrexate - pharmacology, Inhibitory Concentration 50, Flow Cytometry - methods, Drug Screening Assays, Antitumor, Drug Resistance, Neoplasm, Doxorubicin - pharmacology, Cisplatin - pharmacology, Antineoplastic Agents - pharmacology, Tetrazolium Salts - pharmacology, Thiazoles - pharmacology

0 Comments

Editorial

01 October 2022 : Editorial  

Editorial: Rebound COVID-19 and Cessation of Antiviral Treatment for SARS-CoV-2 with Paxlovid and Molnupiravir

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.938532

Med Sci Monit 2022; 28:e938532

SARS-CoV-2/COVID-19

16 September 2022 : Review article  

Effects of Physiotherapy on Rehabilitation and Quality of Life in Patients Hospitalized for COVID-19: A Rev...

Med Sci Monit In Press; DOI: 10.12659/MSM.938141  

03 October 2022 : Clinical Research  

Effect of Vitamin D Concentration on Course of COVID-19

Med Sci Monit 2022; 28:e937741

In Press

30 Sep 2022 : Clinical Research  

Correlation Between Trough Level of Abiraterone and Prostate-Specific Antigen (PSA) Response in Metastatic ...

Med Sci Monit In Press; DOI: 10.12659/MSM.938091  

30 Sep 2022 : Review article  

A Review of the Biological Mechanisms of Dexmedetomidine for Postoperative Neurocognitive Disorders

Med Sci Monit In Press; DOI: 10.12659/MSM.937862  

29 Sep 2022 : Animal Research  

Effect of Antilogous Platelet-Rich Plasma on the Revascularization of Rabbit Prefabricated Flap

Med Sci Monit In Press; DOI: 10.12659/MSM.937718  

26 Sep 2022 : Clinical Research  

Distribution and Determinants of Plasma Homocysteine Levels in a Preconception Population: A Retrospective ...

Med Sci Monit In Press; DOI: 10.12659/MSM.937987  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750