Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

07 August 2010

The protective action of topiramate on dopaminergic neurons

Shengming HuangABCDEF, Haizhen WangABCDEF, Yuming XuDE, Xinyu ZhaoDF, Junfang TengACD, Yunhan ZhangADE

Med Sci Monit 2010; 16(9): BR307-312 :: ID: 881122

Abstract

Background: The present study analyzed the neuroprotective effects of topiramate on 6-hydroxydopamine (6-OHDA)–induced toxicity in primary dopaminergic mesencephalic neuronal cell cultures.
Material/Methods: Through the use of tyrosine hydroxylase and microtubule-associated protein 2 immunocytochemistry, the morphology and development of dopaminergic neurons was observed. Hoechst33258 and propidium iodide (PI) double staining was used to determine cell membrane penetrability and apoptosis by fluorescent microscopy. Methyl thiazole tetrazolium was used to assay cell viability and metabolism. The cells were assigned to 3 groups: (1) control: primary cultured neurons; (2) 6-OHDA: primary cultured neurons and 6-OHDA; and (3) topiramate (TPM) protection: primary cultured neurons and 6-OHDA, treated with various concentrations (10, 50, and 100 µM) of TPM.
Results: Hoechst 33258 and PI double immunofluorescence revealed that the number of dying cells after 6-OHDA treatment was greater than after TPM treatment. Compared with the 6-OHDA group, there were more neurons with greater cell viability in the 6-OHDA plus TPM group.
Conclusions: Topiramate was shown to provide protection to dopaminergic neurons exposed to 6-OHDA by reducing cell apoptosis and enhancing cell viability. The neuroprotective effects of TPM were dose-dependent.

Keywords: Propidium - metabolism, Neuroprotective Agents - pharmacology, Neurons - metabolism, Microtubule-Associated Proteins - metabolism, Fructose - pharmacology, Formazans - metabolism, Fluorescent Antibody Technique, Dopamine - metabolism, Cell Shape - drug effects, Cell Proliferation - drug effects, Cell Death - drug effects, Cell Count, Bisbenzimidazole - metabolism, Staining and Labeling, Tetrazolium Salts - metabolism, Tyrosine 3-Monooxygenase - metabolism

Add Comment 0 Comments

Editorial

01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600

0:00

In Press

06 Mar 2024 : Clinical Research  

Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...

Med Sci Monit In Press; DOI: 10.12659/MSM.941102  

06 Mar 2024 : Clinical Research  

Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943884  

08 Mar 2024 : Clinical Research  

Evaluation of Foot Structure in Preschool Children Based on Body Mass

Med Sci Monit In Press; DOI: 10.12659/MSM.943765  

15 Apr 2024 : Laboratory Research  

The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis

Med Sci Monit In Press; DOI: 10.12659/MSM.943738  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750